• Monitored ill patients during transport to their appointments, and managing medical emergencies
including but not limited to motor vehicle accidents, opioid overdose, and pediatric emergencies.
• Ensured ambulance was properly stocked and functional daily.
• Drafted an estimated 140 patient care reports during this time.

• Maintained and harvested tissue culture samples for the purpose of monitoring
cryptosporidium oocyst population in tissue culture.
• Performed immunofluorescence assays for the purpose of counting cryptosporidium oocysts in
both fecal samples and tissue culture samples.
• Performed intestinal epithelial lymphocyte purification for RNA isolation.
• Assisted in gnotobiotic piglet studies involving cryptosporidium and C. difficile.
• Tracked chemical inventory and updated MSDS’ and performed basic lab management duties.

• Constructed numerous recombinant adeno-associated virus (AAV) vector plasmids.
• Determined concentration of DNA fragments in a NanoDrop and precise molar ratios used to set up
T4 ligase or NEBuilder reactions prior to electroporation into SURE E. coli.
• PCR amplification of DNA inserts for cloning and analysis of plasmids.
• Cultured HEK 293T cells and carried out transfections with lipofectamine to study the functionality
of newly cloned AAV vectors.
• Plasmid purification ranging from minipreps for colony screening purposes and intermediate scale preparations (0.3-2 mg) using QIAGEN Maxi, Mega and Gigapreps.
• Preparation of 20 μm mouse brain cryosections for histological studies.